Biomedical Technology Resources
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Resource Description

The tcaCALC program performs an isotopomer analysis to estimate relative pathway fluxes in biological systems at metabolic and isotopic steady state.  It is used in 13C labeling studies where biological systems (isolated cells, perfused organs or intact animals) are provided enriched substrate(s), and the labeling of intermediates detected using 13C NMR spectroscopy, mass- and tandem mass spectrometry.  The 13C spectra are collected either directly from intact tissue, such as in the case of perfusions conducted inside a magnet, or after extraction of a tissue sample (isolated cell studies or tissue samples taken after in vivo infusion of 13C-enriched substrates)


Isotopic Signatures, Isotopomer Analysis, Mass Spectrometry