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Resource for NMR Molecular Imaging of Proteins

Stanley Opella, Ph.D.
P41EB002031
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Center Overview

The Resource is focused on the development of NMR spectroscopy for structure determination of proteins in biological supramolecular assemblies. The principal applications are to membrane-associated proteins; however, the approach is generally applicable to polypeptides that cannot be prepared in forms suitable for X-ray crystallography or multidimensional solution NMR spectroscopy. As a result, there are also applications to viruses and other biological systems.The principal instrumentation consists of high-field NMR spectrometers dedicated to high-resolution solid-state NMR spectroscopy. The spectrometers are capable of the full-range of multiple-resonance experiments on stationary and spinning samples; however, the major emphasis is on methods that utilize mechanically or magnetically oriented samples. Development encompasses preparation of samples, including:

  • Expression and purification of membrane proteins
  • Design and construction of instrumentation, especially probes
  • Implementation of new pulse sequences and other experimental protocols for solid-state NMR spectroscopy
  • Calculations for the processing of experimental data and protein structure determination from the orientational constraints derived from these data.